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ORIGINAL ARTICLE
Year : 2014  |  Volume : 2  |  Issue : 2  |  Page : 28-32

Detection of intestinal protozoa by using different methods


1 Department of Parasitology, Faculty of Medical Technology and Engineering, Sebha University, Barack, Libya
2 Department of Dermatology, Era's Medical College and Hospital, Lucknow, India
3 Department of Pediatrics, Jawaharlal Nehru Medical College, Aligarh, Uttar Pradesh, India
4 Department of Parasitology, Faculty of Medicine, Sebha University, Barack, Libya

Correspondence Address:
Abdul Hafeez Khan
Department of Parasitology, Faculty of Medicine, Sebha University, Sebha
Libya
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2348-1471.143326

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A total of 305 stools samples during the period of October 2011 to June 2012 were collected from patients attending Brack General Hospital and Medical Technology, Department of Brack, Al-Shati, Libya. All stool samples were examined by direct smear preparation in normal saline, iodine, and eosin stains and 4 concentration methods (formalin-ether, normal saline sedimentation, zinc sulfate, and Sheather's sugar flotation). Of the 305 samples, 18.03% stools were found positive for protozoan parasites in direct smear microscopy. Normal saline sedimentation and zinc sulfate flotation detected 27.21% and 23.6% positive samples, respectively. However, formalin-ether was found to be the most sensitive method. Sheather's sugar flotation failed to detect Entamoeba histolytica/dispar, Entamoeba coli, and Blastocystis hominis. B. hominis was the most common parasite among the patients. Concentration techniques showed significantly higher (P < 0.05) detection rates of parasites compared with direct smear microscopy.


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